台北中医医学杂志

96.01 山楂成分保护PC12 细胞之作用
山楂成分保护PC12 细胞之作用
Crataegus Pinnatifida Component Protect PC12 Cell Effect
陈华鑫1 张嘉麟2* 林博楷2
1 澄清综合医院中港分院药剂部
2 弘光科技大学生物科技系(所)
【摘要】
帕金森氏症是一种神经退化性疾病,近年来已有文献报导巴拉刈与诱导帕金森氏症的产生有关。大鼠肾上腺髓质嗜铬细胞瘤细胞株(PC12 cellline)在神经分化与细胞死亡研究方面被普遍使用,因此PC12 细胞可以作为神经保护作用的研究模式。山楂具备抗癌、抗氧化及抗发炎等多种生理活性,本研究主要是探讨山楂成分抑制巴拉刈诱导PC12 细胞死亡的作用,结果得知山楂95% 乙醇萃取物及山楂酸抑制巴拉刈毒性的能力最佳,可以保护PC12 细胞,未来将探讨山楂成分保护PC12 细胞作用机制,期盼山楂成分能成为神经保护剂。
【关键词】巴拉刈、PC12 细胞、山楂
 
Chen Hua-Sin1 Chang Chia-Lin2* Lin Po-Kai2
2Research Institute of Biotechnology, HungKuang University
【Summary】
Parkinson’s disease (PD) is a neurodegenerative disorder. In recent years, it has been reported in the literature that paraquat is associated with PD production.The rat adrenal medullary pheochromocytoma cell line (PC12 cell line) is a commonly used model for studies of neural differentiation and cell death. PC12 cells can be used a study model of neuroprotective effect. Crataegus pinnatifida has many physiological activities such as anti-cancer, anti-oxidant and anti-inflammatory effects. This study is mainly to investigate the C. pinnatifida component inhibit paraquat-induced PC12 cell death effect. It is found the 95% ethanolic extract of C. pinnatifida and the crataegolic acid have the best ability to inhibit the toxicity of paraquat. They can protect PC12 cells. In the future, we will research the protective mechanism of action of PC12 cells of the C. pinnatifida component. It is expected that the C. pinnatifida component can become a neuroprotective agent.
【Keywords】Paraquat, PC12 Cell, Crataegus Pinnatifida